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KMID : 0604520140400020163
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Journal of the Society of Cosmetic Scientists of Korea
2014 Volume.40 No. 2 p.163 ~ p.170
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Effects of Residual Solvents in the Phase Transition, Transition Enthalpy, and Transition Temperature of Phospholipid Membranes
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An Eun-Seol
Choi Jae-Sun
Lee Dong-Kuk
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Abstract
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Lipid membranes composed of phosphatidylcholine (PC) are used in biophysical study to mimic cellular mem-branes and interactions between the membrane and chemicals, where organics solvents are used in dissolving lipids or chemicals. Later, solvents are removed from the solution under nitrogen gas at room temperature, followed by the further removal of the solvent at vacuum condition for several hours. In this process, some solvents are easily removed under described conditions above and others are required more severe conditions. In this study, 31P solid-state nuclear magnetic resonance (SSNMR) techniques and differential scanning calorimetry (DSC) were used to see any changes in the line shapes of 31P NMR spectra of multilamellar vesicles (MLVs) samples of POPC and in the phase change temperature of multilamellar vesicles (MLVs) of DPPC in DSC thermogram with or without any residual solvents. The thermody-namic parameters associated with the solvents did exhibit noticeable changes depending on solvent types. Thus, it is concluded that solvents should be carefully chosen and removed completely and experimental results should also be interpreted with caution particularly for the experiments investigating lipid phase changes and related topics.
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KEYWORD
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Lipid phase, Differential Scanning Calorimetry, DSC, lipid membrane, Solvent effects, 31P solid-state Nuclear Magnetic Resonance (31P SSNMR).
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